Fig. 7.

Icariside I/LPS induces liver injury in WT mice but not in NLRP3 inflammasome knockdown mice. a-f  WT female C57BL/6 mice were given 50 mg/kg MCC950 or its saline vehicle through intraperitoneal injection. Then, mice were pretreated with LPS (2 mg/kg) through the tail vein. 2 h later, intraperitoneally Icariside I (50 mg/kg n = 6) injection was conducted. 6 h after Icariside I injection, serum levels of ALT (a), AST (b), IL-1β (c), TNF-α (d) were measured by assay kit, (e, f) IL-1β (e) and caspase-1 activity (f) in the livers was detected after BCA protein quantification and normalization processing. g H&E staining. Data are expressed as the mean ± S. ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. the control. *P < 0.05, **P < 0.01, ***P < 0.001