Fig. 5.

Binding kinetic analysis of NiV-G to wt or mutant L124A ephrin-B2 by bio-layer interferometry. (a) Real-time binding analysis of NiV-G to the wt or mutant L124A ephrin-B2. Bio-layer interferometry was used to measure the binding kinetics of soluble NiV-G to both ephrin-B2 (wt) and its mutant (L124A) in response units (nm). Biotinylated soluble NiV glycoprotein G (sNiV-G) was immobilized to streptavidin-coated biosensors, and the binding of wt and mutant ephrin-B2 was assessed at the indicated concentrations. (b) Steady-state analysis of real-time binding data obtained with the Octet Red system. The plots in each panel show response versus protein concentration curves derived from the raw binding data. One representative experiment of two is shown.