Table 2. Infection types (ITs) on seedlings (2016) and infection responses (IRs) on adult plants (2016–2017), and amplification results for the known Sr genes by markers.
| Line | Pidegree | 21C3CTTTM | 34C0MRGSM | 34C3MTGQM | Sr gene | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| ITs | IRS | ITs | IRS | ITs | IRS | ||||||
| 2016 | 2016 | 2017 | 2016 | 2016 | 2017 | 2016 | 2016 | 2017 | |||
| Nongda09-1818 | Nongda03-2379/Nongda02-4339 | 0 | I | I | 0 | I | 5R | 1 | 10R | 5R | – |
| Nongda07-1328 | Longmai26/Nongda93-5233 | 1 + | 10R | I | ; | 5R | 10R | 0 | I | I | 38 |
| Nongda08-1639 | Nongda01-4576/Nongda01S-1197 | 0 | I | I | 0 | 5R | 5R | 0 | I | 5R | – |
| Nongda09-1884 | Longjian03-062/Nongda01-4551 | 0 | I | I | 0 | I | I | 0 | I | I | – |
| Jiusan07-6378 | Jiusan00-6709/Jiusan00-5006 | 0 | I | I | 0 | I | I | 0 | I | I | 31 |
| Jiusan07-6086 | Jiusan01F4-666/Kehan19 | 0 | 5R | 5R | 0 | I | I | 0 | I | I | 38 |
| Jiusan07-7106 | Jiusan01F3-889/Jianmai411 | ;1- | 10R | 10R | 0 | I | I | ;1 | 10R | 10R | – |
| Jiusan07-7409 | Beimai6/Jiusan01-6111 | 0 | I | I | 0 | I | I | 0 | I | I | 38 |
| Jiusan07-5221 | Nongda1662/Beimai6 | 0 | I | I | 0 | I | I | 0 | I | I | 38 |
| Jiusan07-7395 | Jiamai411/Jiusan99-6135 | 1 | 10R | 10R | 0 | 5R | 5R | 0 | I | I | – |
| Jiusan06-6203 | Jiusan01F3-889/Jiamai411 | 1 | 5R | 10R | 1 + | 10R | 20R | 2 | 10R | 30R | 31 |
| Jiusan07-6205 | Longmai30/Jiusan98-61249 | 3 | 30MS | 30S | 1 | 40R | 30R | 1 + | 30MR | 30R | – |
| Gang07-151 | Kenhong17/Gang94-441 | 0 | I | I | ; | 5R | I | ; | 5R | 5R | – |
| Gang09-558 | Longmai26/Gang94-445//Longfu990761 | 0 | I | I | ; | 20R | 20R | 0 | I | I | 38 |
| Pinzi66087 | CROC-1/A.SQ//2*OPATA/3/97-7293/4/94-4081 | 0 | I | I | ;1 | 20R | 30R | 0 | I | I | – |
| Pinzi96563 | N4/T.dicoccoides CWI19167 | 0 | I | 5R | 0 | I | I | ; | 5R | 5R | – |
| Pinzi86119 | Wangshuibai/Longmai26//Longmai19 | 0 | I | I | 0 | I | I | 0 | I | I | 2 |
| Pinzi96860 | Xinkehan9/SYN333//Longmai26 | 1 | 5R | 10R | ;1- | 5R | 20R | 1 | 20R | 20R | - |
| Pinzi96029 | ALTAR84/A.SQ(224) (R.F.)//97-7137/3/97-7146 | ; | 5R | 10R | 0 | I | I | 0 | I | I | – |
| Pinzi96864 | Xinkehan9/SYN333(R)//Longmai26 | ;1- | 10R | 10R | ;1- | 5R | 10R | 0 | I | I | - |
| Pinzi96248 | DR147/T.TIMOPHEVII CWI 17006//Longmai26 | 0 | I | I | 0 | I | I | 0 | I | I | 2 |
| Longfu05-0281 | 96RF6 1199qun/Longmai26 | 0 | I | I | 0 | I | I | 0 | I | I | 2, 38 |
| Longfu08-6247 | Fengshi001/Ke90-514 | 0 | I | I | 1 | 10R | 5R | 0 | I | I | – |
| Longfu08-586 | Kefeng10/Long00-0117 | 0 | I | I | ;1 | 5R | 10R | ; | 5R | 5R | 38 |
| Longfu09-1176 | Kang1396/Zhongyuan9//Kefeng10 | ; | 5R | 5R | 1- | 10R | 10R | ;1 | 10R | 20R | 38 |
| Longfu09-1249 | Kang151-4/Ke85-858//Keyin5 | 2 | 20R | 30R | 1 | 10R | 10R | 3- | 30MS | 30MS | – |
| Longfu09-0594 | Long00-0657/Shandong5-27 | 0 | I | I | 0 | I | I | 0 | I | I | – |
| Jiusan07-7371 | Jiusan01F3-889/Jiamai411 | 1 + | 30R | 30R | 3 | 50S | 60S | 4 | 70S | 80S | – |
| Jiusan07-6086 | Jiusan01F4-666/Kehan19 | 1 − | I | I | 0 | I | I | 1 + | 5R | 10R | 31, 38 |
| Jiusan09u294 | JinK1638/Jiusan05yun40 | 1 | 10R | 20R | 0 | I | I | 1 | 20R | 20R | – |
| Hong09-556 | Gang03-441/M306 | 4 | 70S | 70S | 3 | 50S | 60S | 3- | 60S | 60S | – |
| Gang07-151 | Kenhong17/Gang94-441 | 0 | I | I | 0 | I | I | 0 | I | I | – |
| Gang09-557 | Longmai26/Gang94-445//M306 | 1 | 10R | 10R | 0 | I | I | 1 | 50R | 40MR | – |
| Gang06-4 | Beimai2/Yuan96-3 | 4 | 70S | 70S | 3 | 50S | 70S | 4 | 40S | 90S | – |
| Hong10-598 | Long01-1122/Nongda97-2829 | 0 | I | I | 0 | I | I | 0 | I | I | 2, 38 |
| Hong10-614 | Nongda00-1667/Long01-1237 | 0 | I | I | 0 | I | I | 2 | 20R | 10R | – |
| Hong10-595 | Long01-1122/Longmai00-4379 | 0 | I | I | 0 | I | I | 0 | I | I | – |
| Hong09-558 | Gang03-441/Longmmai990761 | 0 | I | I | 1- | 5R | 5R | 4 | 40S | 40S | – |
| Hong09-552 | Gang03-441/M306 | 2 | 10R | 10R | 0 | I | I | 1 + | 30MR | 30R | – |
| Hong10-600 | Long01-1122/Nongda97-2829 | 0 | I | I | ;1 | 20R | 20R | 1 | 10R | 20R | – |
| Nong10-0853 | Jiuyumai23/Le89-446//Long01-1237 | 0 | I | I | 0 | I | I | ; | 5R | 5R | – |
| Nong10-0313 | Long03F3-6519/Longfu20-378 | 1- | 30R | 30R | 0 | I | I | 1 | 10R | 30R | – |
| Nong10-0449 | Huapei3901/Jiusan62504//Longfu03Chanjianbeibao1 | 0 | I | I | 1 | I | I | 1 | 5R | 5R | 31 |
| Nong09-9702 | Longmai26/Long02-2165 | 0 | I | I | 0 | I | I | 1 | 10R | 30R | 38 |
| Nong10-0631 | Long04-4370/Long02-2309 | 0 | I | I | 0 | I | I | 0 | I | I | 2, 38 |
| Nong10-0333 | Long03F3-6519/Longfu20-378 | 0 | I | I | 0 | I | I | 1 + | 30R | 30R | – |
| Nong10-7767 | Long94-4081/Xiaobing32 | 0 | I | I | 0 | I | I | 0 | I | I | 31 |
| Nong10-0150 | Ke89-446/Jiamai6 | ;1 | 30R | 30R | 0 | I | 5R | 2 | 40MR | 20MR | – |
| Nong10-0632 | Long04-4370/Long02-2309 | ;1- | 10R | 5R | 2 | 30R | 20R | 2 | 30R | 30R | – |
| Nong11-1540 | (Huapei3901/Jiusan62504)F .1/Fancai | 0 | I | I | 1 | 20R | 20R | 1 + | 40R | 40R | – |
| Nong11-1530 | (Huapei3901/Jiusan62504)F .1/Hebai9413 | 0 | I | I | 1 + | 10R | 10R | 2 | 20R | 20R | – |
| Nong11-1393 | (Longmai26/Long98-8906)F .1/Long2003M8059-3 | ;1 | 10R | 10R | 0 | I | I | 1 | 10R | 20R | 2, 38 |
| Nong11-1664 | [(Longmai26/Zhouzhou9023)/Longmai29]F .1/Fancai | 0 | I | I | ;1 | 5R | 10R | 1 + | 30R | 30R | 2 |
| Nong11-1494 | (Long03-3152/Jiusan62504)F .1/Fancai | 0 | I | I | 0 | I | I | 2 | 40MR | 40MR | – |
| Nong08-8830 | 91-1178/Yemao | 0 | I | I | 0 | I | I | 0 | I | I | – |
| Nong11-1789 | 97-7215/95-3577//Longmai26 | 0 | I | I | 0 | I | I | 2 | 5R | 10R | 2, 38 |
| Nong10-0453 | Huapei3901/Jiusan62504//Longfu03Chanjianbeibao1 | 0 | I | I | 0 | I | I | 0 | I | I | 31 |
| Nong11H1336 | Jiusan99-5611/Kefeng6 | 0 | I | I | 0 | I | I | 2 | 20R | 20R | 2, 31 |
| Nong11za136 | Huapei3901/Jiusan62504//Longfu20-378 | 1 + | 20R | 10R | 1 | 20R | 20R | 2 | 30R | 20R | – |
| Nong10-0852 | Jiuyubai23/Ke89-446//Long01-1237 | 0 | I | I | 0 | I | I | 1 | 10R | 20R | – |
| Nong10-0149 | Ke89-446/Jiamai6 | 1 + | 30R | 30R | 1 | 30R | 40R | 1 + | 40R | 50R | – |
| Nong11-2097 | Ke90-513/Longmai30 | 1 | 10R | 10R | 0 | I | I | 1 | 5R | 5R | 31, 38 |
| Nong10-0995 | Ke93-387/Long99-9126//Long01-1237 | 0 | I | I | 0 | I | I | 1 | 10R | 5R | – |
| Nong06F6-5299 | Kefeng6/Liupeiti//83199/3/3901 | 2 | 30MR | 30MR | 1 | 20MR | 20MR | 1- | 20R | 20MR | – |
| Nong10-0716 | Long03-3152/Jiusan62504 | 0 | I | I | 0 | I | I | 0 | I | I | 38 |
| Nong11-1432 | Long03-3651/Longfu93-217//Longmai26 | 1 | 10R | 10R | 0 | I | 5R | 1 | 10R | 10R | 2 |
| Nong11-2110 | Long03-3675-1/Longfubeibaochanjian1 | 3 | 20MS | 20MS | 1 | 10MR | 20MR | 4 | 40MS | 40S | – |
| Nong10-0334 | Long03F3-6515-19/Longfu20-378 | 1 | 20R | 20R | ;1 | 5R | 10R | 2 | 10R | 40R | – |
| Nong10-0629 | Long04-4370/Long02-2309(Ke165-3/Longfu10) | 0 | I | I | 0 | I | I | 1 | 5R | 20R | 38 |
| Nong11-2294 | Long04-4370(Kefeng6/Long94-4081)/Long02-2309 | 0 | I | I | 0 | I | I | 1 | 10R | 20R | – |
| Nong11-2289 | Long04-4370/Long02-2309(Ke165-3/Longfu10) | 0 | I | I | 0 | I | I | 0 | I | I | 38 |
| Nong06-7721 | Long91-1131/Long94-4081 | ;1 | 5R | 10R | ; | 10R | 10R | 1 | 20R | 20R | – |
| Nong11-1062 | Long94-4081/Long97-7146(Longmai30) | ;1 | 5R | 20R | 1- | 20R | 20R | 1 + | 20R | 20R | 38 |
| Nong10-0509 | (Longfumai10/Longmai26)/Kefeng5//Long01F3-5050-1 | 0 | I | I | 0 | I | I | 1 + | 40MR | 40R | 2 |
| Nong10-0518 | Long99-6189-2/Kefeng5//Long01F3-5050-1 | 0 | I | I | 0 | I | I | 1 | 20R | 20R | – |
| Nong11-1056 | Longmai26/Kefeng4 | 0 | I | I | ; | 10R | 10R | 1 | 10R | 5R | 38 |
| Nong11-1017 | Longmai26/Lelao6 | 0 | I | I | ; | 5R | 5R | 1 | 30R | 30R | 2 |
| Nong11-1027 | Longmai26/(Kefeng5/Xiaobing33//Longmai26) | 0 | I | I | 0 | I | I | 0 | I | I | 2, 31 |
| Nong10-0070 | Longmai26/Longmai30 | 0 | I | I | 0 | I | I | ;1 | 10R | 5R | 38 |
| Nong11-1074 | Longmai30/Kelao6 | 2 | 30R | 30R | 0 | I | I | 2 + | 50MR | 40MR | – |
| Nong11H1029-2 | Qi565-1*Long03-3651 | 0 | I | I | 0 | I | I | 1 | 30R | 30R | – |
| Nongda10-2001 | Nongda95-1743/Nongda93-5223 | 0 | I | I | 0 | I | I | 1 | 10R | 10R | – |
| Nongda10-1199 | Nongda04-2144/Nongda02-4541 | ;1 | 5R | 10R | 1- | 5R | 5R | 1 | 5R | 5R | – |
| Nongda07-1328 | Longmai26/Nongda93-5233 | ; | I | I | 0 | I | I | ; | I | I | 2, 38 |
| Longfu09-534 | (Kefeng10/Ke95RF-1750)F .0 | 0 | I | I | 0 | I | I | 3 + | 50MS | 40S | – |
| Longfu10K329 | Jiusankang151-6 Mutagenic Line | 0 | 5R | 5R | 1 | I | I | 1 | 10R | 10R | 31 |
| Longfu10-848 | Longfu02-12518/03K604 | 1 | 5R | 10R | ; | I | I | 1 + | 30R | 30R | – |
| Longfu10-367 | Long0657/Jiusan3U108 | 4 | 70S | 80S | 4 | 80S | 80S | 4 | 90S | 80S | – |
| Longfu10-797 | Gang03Jian912/01-4379 | 1 | 5R | 5R | 0 | I | I | ;1 | 30R | 30R | – |
| Longfu10-683 | Long6239-CH5R-2/Kefeng10 | ; | I | 10R | 0 | I | I | ;1 | 20MR | 20R | 2 |
| Longfu10-527 | Kefeng10/Ke96RF6-976//Ke95R498SP4 | 0 | I | I | 0 | I | I | 0 | I | I | 38 |
| Longfu11-243 | 04-711/Beiyin01-4 | 1- | 5R | 5R | 0 | I | I | ;1 | 30R | 30R | – |
| Longfu09-358 | (Ke88-418/Shandong95-9195)F .0 Mutagenic Line | 1 | 20R | 30R | 1 | 20R | 40R | 2 | 10R | 30R | – |
| Longfu08-6564 | Kefeng10 Mutagenic Line /Long00-0117 | 0 | I | I | 0 | I | I | 0 | I | I | 31, 38 |
| Longfu10-891 | Longfu01-4379/Kefeng9 | 1 | 10R | 10R | ; | 5R | 5R | 1 + | 30R | 30R | – |
| Little Club | – | 4 | 90S | 90S | 4 | 90S | 100S | 4 | 100S | 100S | – |
Notes.
a
IT: infection types scored in the greenhouse seedling tests were based on a 0-to-4 scale (Stakman, Steward & Loegering, 1962) where ITs, 0, 1, or 2 were considered resistant and ITs 3 or 4 susceptible; and symbols + and –indicated slightly larger and smaller pustule sizes, respectively.
b
IR: Infection responses were scored at the adult plant stage in the field tests following the descriptions of Roelfs, Singh & Saari (1992), where I = immune, R = resistant, MR = moderately resistant, MS = moderately susceptible, and S = susceptible.