Figure 6.

(A) Anti-Hpa2 mAbs neutralize the scattering capacity of Hpa2. RT4 cells were allowed to establish colonies and were then treated with Hpa2 (10 µg/ml) in the presence of control mouse IgG or the indicated anti-Hpa2 mAb (30 µg/ml). Colony morphology was examined after 24 h. Shown are representative images at x100 (original magnification). (B) Immunofluorescent staining. SIHN-013 cells were left untreated (Control; upper panels) or were incubated with Hpa2 (5 µg/ml) for 30 min (lower panels). Cells were then washed, fixed, and subjected to immunofluorescent staining applying anti-Hpa2 (20c5; green) and anti-syndecan-1 (red) antibodies. Merge images are shown in the right panels. Note co-localization of Hpa2 and syndecan-1 on the cell membrane.