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. Author manuscript; available in PMC: 2021 Jun 17.
Published in final edited form as: Neuron. 2020 Apr 21;106(6):992–1008.e9. doi: 10.1016/j.neuron.2020.03.025

Figure 2. Loss of NFIA impairs calcium activity in hippocampal astrocytes.

Figure 2.

(A–P) Representative images and traces from AA6-Con and AA6-KO mice showing spontaneous GCaMP6s activity in astrocytes from olfactory bulb, cortex, brainstem, and hippocampus. Red line denotes soma and blue line depicts approximate territory; traces are from soma (Q,R) Quantification of somatic DF/F GCaMP6s signal amplitude (Q) and frequency (R) derived from 3 mice from each genotype, total cells per region: OB = 15, CX = 15, BS = 10, HP = 12. (S) representative tracks of body positions (upper panels) and zone preference index (lower panel) showing odor detection threshold; data were derived from 12 AAG-Con and 11 AAG-KO mice. Two-tailed unpaired t-test under each (R)-Limonene concentration. (T) 2-min average breathing rate (upper panel) and tidal volume (lower panel) over 60 min testing period; data were derived from 12 AAG-Con and 12 AAG-KO. Two-way repeated measures ANOVA. (U) Quantification of freezing in contextual fear conditioning (upper panel) and novel place recognition (lower panel). In upper panel, 19 AAG-Con and 20 AAG-KO mice were used; in lower panel, 13 AAG-Con and 13 AAG-KO mice were used. Two-tailed unpaired t-test. OB: olfactory bulb; CX: cortex; HC: hippocampus; BS: brainstem. AA6-Con denotes: NFIA+/+; Aldh1l1-CreER; Rosa-CAG-LSL-GCaMP6s. AA6-KO denotes: NFIAfl/fl; Aldh1l1-CreER; Rosa-CAG-LSL-GCaMP6s. AAG-Con denotes: NFIAfl/fl; Aldh1l1-GFP. AAG-KO denotes: NFIAfl/fl; Aldh1l1-CreER; Aldh1l1-GFP. *p< 0.05, **p<0.01. Also see Figure S4–S5.