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. Author manuscript; available in PMC: 2021 Jun 17.
Published in final edited form as: Neuron. 2020 Apr 21;106(6):992–1008.e9. doi: 10.1016/j.neuron.2020.03.025

Figure 7. Inhibition of LTP in mice lacking astrocytic NFIA is dependent upon Ca2+ activity.

Figure 7.

(A) Schematic of LTP recording experiment. (B) Schematic of AAV-GFAP-GqDREADD rescue experiment. (C) LTP traces from AAG-Con and AAG-KO hippocampal slices. (D) LTP traces from AAG-Con and AAG-KO hippocampal slices infected with AAV-GFAP-GqDREADD. (E,F) Representative traces of evoked NMDAR current before and after treatment of DCKA (e) and D-serine (F) from AAG-Con and AAG-KO hippocampal slices. (G) Quantification of DCKA or D-serine sensitive evoked NMDAR current measured at 50 ms after stimulation. (H) LTP traces from AAG-Con and AAG-KO hippocampal slices supplemented with D-serine. (J) Quantification of LTP. All electrophysiological experiments is derived 3 mice from each genotype, ranging from 7–13 cells total for each experiment. * p< 0.05, ***p<0.001, Student’s two-tailed paired (G) and unpaired (J) t-test. AAG-Con denotes: NFIAfl/fl; Aldh1l1-GFP. AAG-KO denotes: NFIAfl/fl; Aldh1l1-CreER; Aldh1l1-GFP. Also see Figure S8.