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. Author manuscript; available in PMC: 2021 Aug 13.
Published in final edited form as: Horm Metab Res. 2020 Aug 13;52(8):607–613. doi: 10.1055/a-1212-8803

Figure 2. Orthogonal validation of differential gene expression identified by targeted next-generation RNA sequencing (RNAseq) of archival formalin-fixed paraffin-embedded (FFPE) adrenal tumor tissue.

Figure 2.

(A and C) Box plots of log2-normalized CYP11B2 and IGF2 expression values generated from targeted RNAseq data highlight differential gene expression across adrenal tumor types [adrenal cortical carcinoma (ACC), cortisol-producing adenoma (CPA), aldosterone-producing adenoma (APA)] (P < 0.001), which is confirmed by quantitative reverse transcription PCR (qRT-PCR) analysis (P < 0.001). (B and D) Scatter plots of paired RNAseq- and qRT-PCR-derived log2-normalized CYP11B2 and IGF2 expression values demonstrate high correlation across adrenal tumor types (Pearson correlation coefficient > 0.92; P < 0.001). ACC = yellow, CPA = blue, and APA = red.