Figure 2.

Single-nucleus RNA-seq reveals cell-specific transcriptional response to cocaine. a, Global dimensionality reduction clustering (UMAP) of 15,631 individual NAc nuclei identifies 16 transcriptionally distinct cell classes of the rat NAc, including MSNs expressing Drd1 and Drd2 mRNA. b, Circos plot of differentially expressed genes 1 h after cocaine administration, analyzed within each cell type. Cocaine differentially expressed genes are most abundant in Drd1-MSNs, followed by astrocytes. c, UMAP plot from a in gray with the representative expression of Fosb, a cocaine-responsive immediate-early gene. Fosb mRNA expression is increased in a small fraction of “activated” Drd1-MSNs. Data from Savell et al. (2020).