Figure 1.

ChABC-induced plasticity requires intact TRKB signaling in PV⁺ neurons. A, PNN removal by local chABC injection reopens critical period-like plasticity in the visual cortex of WT but not in mice heterozygous for TRKB deletion in PV interneurons (PV-TRKB^+/−), as measured by optical imaging of the intrinsic signal before (IOS1) and after 7 d of MD (IOS2). ODI: ocular dominance index. B, ChABC treatment (30 min) of cortical neurons (7 DIV) increases pTRKB. C, The CSPG aggrecan, added to cortical neurons (6 DIV) for 24 h, attenuates BDNF-induce pTRKB in cortical neurons. Columns and bars represent mean ± SEM, respectively, and scattered points represent individual values. Data were analyzed by three-way ANOVA and Tukeys's post hoc (A), Mann–Whitney (B), or two-way ANOVA followed by Tukey's multiple comparison test (C); *p < 0.05, **p < 0.005, ***p < 0.0005.