Figure 3.

Fluoxetine disrupts TRKB and PTPσ interaction in vitro and in vivo. A, Fluoxetine (FLX) disrupts the interaction between TRKB and PTPσ in 7 DIV cortical neurons, and (B) in the visual cortex (V1) of mice systemically treated (30 mg/kg, i.p.) 30 min before tissue dissection. C, Fluoxetine does not affect the detection of PTPσ extracellular domain on the cell surface, indicating no effect on PTPσ surface exposure or PTPσ ectodomain shedding. D, Mutation of the TRKB transmembrane domain (R427A/Y433F) partially disrupts the TRKB:PTPσ interaction and abolishes the effects of fluoxetine on it. Columns and bars represent mean ± SEM, respectively. Data were analyzed by one-way ANOVA followed by Bonferroni's (A), two-way ANOVA (D), or unpaired t test (B, C); *p < 0.05, **p < 0.005.