Table 1.
Statistical analysis and the number of animals/samples used in the experiments
| Graph | Pre hoc | Post hoc | N, number of samples/animals per group |
|---|---|---|---|
| 1A | Three-way ANOVA (time × genotype × drug): General effect: Time (IOS): F(1,34) = 4.139, p = 0.0498* Genotype: F(1,34) = 0.7739, p = 0.3852 chABC: F(1,34) = 5.164, p = 0.0295* Interaction: Time × genotype: F(1,34) = 4.861, p = 0.0343* Time × chABC: F(1,34) = 6.483, p = 0.0156* Genotype × chABC: F(1,34) = 7.244, p = 0.0110* Time × genotype × chABC: F(1,34) = 5.899, p = 0.0206* |
Tukey's multiple comparison test: WT-chABC (IOS1) × WT-chABC (IOS2): p = 0.0002* |
WT-vehicle: 3 (we have used the lowest number of animals possible in the control group to reduce the total number of animals used in the experiments; there is abundant evidence of no shift in ocular dominance in adult WT mice both from our lab and in the literature) WT-chABC: 6 PV-TRKB+/−-vehicle: 5 PV-TRKB+/−-chABC: 7 |
| 1B | Mann–Whitney: U = 0, p = 0.0286* | Vehicle: 4 chABC: 4 |
|
| 1C | Two-way ANOVA: Interaction: F(1,20) = 3.921, p = 0.0616 BDNF: F(1,20) = 155.2, p < 0.0001* Aggrecan: F(1,20) = 5.117, p = 0.035* |
Tukey's multiple comparison test: BDNF-vehicle × BDNF-aggrecan: p = 0.0329* |
Vehicle-vehicle: 6 Vehicle-aggrecan: 6 Vehicle-BDNF: 6 BDNF-aggrecan: 6 |
| 2B | Unpaired t test: T22 = 4384, p = 0.0002* | WT: 12 PTPσ+/−: 12 |
|
| 2C | One-way ANOVA: F(2,14) = 91.44, p < 0.0001* |
Tukey's multiple comparison test: WT × PTPσ+/−: p = 0.0007* WT × PTPσ-/-: p < 0.0001* PTPσ+/− × PTPσ-/-: p < 0.0001* |
WT: 5 PTPσ+/−: 6 PTPσ-/-: 6 |
| 2D | Mann–Whitney: U = 0, p = 0.0002* | WT: 7 PTPσ+/−: 9 |
|
| 2E | Mann–Whitney: U = 9, p = 0.0164* | WT: 7 PTPσ+/−: 9 |
|
| 2F | Two-way ANOVA: Interaction: F(1,8) = 33,13, p = 0.0004* Time: F(1,8) = 40.81, p = 0.0002* Genotype: F(1,8) = 36.70, p = 0.0003* |
Bonferroni's multiple comparison test: IOS1-PTPσ+/− × IOS2- PTPσ+/−: p = 0.0001* |
WT: 6 PTPσ+/−: 4 |
| 3A | One-way ANOVA: F(2,15) = 6.734, p = 0.0082* |
Bonferroni's multiple comparison test: vehicle × fluoxetine 1 μm: p = 0.0046* |
Vehicle: 6 Fluoxetine 0.1 μm: 6 Fluoxetine 1 μm: 6 |
| 3B | Unpaired t test: t(6) = 4.025, p = 0.0069* | Vehicle: 4 Fluoxetine: 4 |
|
| 3C | Unpaired t test: t(28) = 0.9872, p = 0.3320 | Vehicle: 15 Fluoxetine: 15 |
|
| 3D | Two-way ANOVA: Interaction: F(1,19) = 0.8761, p = 0.3610 Fluoxetine: F(1,19) = 2.627, p = 0.1216 TRKB mutation: F(1,19) = 5.131, p = 0.0354* |
WT-TRKB-vehicle: 6 WT-TRKB-fluoxetine: 6 Mutant TRKB-vehicle: 5 Mutant TRKB-fluoxetine: 6 |
*Statistically significant.