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. 2021 Feb 15;131(4):e139496. doi: 10.1172/JCI139496

Figure 3. Skeletal muscle KLF15 is a major regulator of lipid flux pathways.

Figure 3

(A) Heatmap clustering of 496 differentially enriched genes found in RNA sequencing of soleus muscle (n = 4). (B) Gene-set enrichment analysis using Gene Ontology Biological Processes showing the top 20 most significantly enriched pathways. (C) qPCR of select lipid flux pathway genes in soleus (n = 3–7). (D) Acylcarnitine analysis in soleus by tandem mass spectrometry. Data normalized to control group average for each acylcarnitine species and significantly different species (P < 0.05) are shown (n = 6–9). (E) Metabolic cage respiratory exchange quotient (RER) (n = 8–9). (F) Schematic representation of lipid flux pathway and defects seen in K15-SKO mice. Data represent mean ± SEM. Comparisons between MyoCre and K15-SKO mice were performed using an unpaired, 2-tailed Student’s t test, *P < 0.05, **P < 0.01, ***P < 0.001.