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TRIM14 was a target gene of miR-198. (A) The predicted target region of TRIM14 3’UTR with the miR-198 binding sites and mutant binding sites was shown. (B) Dual-luciferase reporter assay was used to detect the interaction between miR-198 and TRIM14 in U138/TMZ and LN18/TMZ cells. (C, D) qRT-PCR and WB analysis were used to measure the effect of miR-198 expression on the mRNA and protein expression of TRIM14. ***P< 0.001.
