Table 2. Primer sequences for RT-qPCR.
Forward (5’-3’) | Reverse (5’-3’) | |
miR-138 | GCCGCAGCTGGTGTTGT GAAT | GCGAGCACAGAATTAATACGAC |
GAPDH | ACCACAGTCCATGCCATCAC | TCCACCACCCTGTTGCTGT |
IL-1β | GACTTCACCATGGAACCCGT | GGAGACTGCCCATTCTCGAC |
U6 | CTCGCTTCGG CAGCACA | GCGAGCACAGAATTAATACGAC |
FBLN5 | ACTGAAGGGGGTTAAGCGAAA | GAGGGATGCACAGATACCCG |
elastin | GCCATTCCTGGTGGAGTTCCTGGA | ACCGCACCTGCAGACACTCCTAAG |
Note: RT-qPCR, reverse transcription quantitative polymerase chain reaction; miR-138, microRNA-138; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IL-1β, interleukin-1β; FBLN5, fibulin-5.