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. 2020 Dec 11;13(2):2655–2667. doi: 10.18632/aging.202309

Figure 2.

Figure 2

Fucoxanthin down-regulated the expression of COX-2 and iNOS in LPS-activated RAW 264.7 macrophages. (A) RAW264.7 cells were pretreated with the indicated concentrations of Fucoxanthin for 1 h before being stimulated with LPS for another 24 h. Cox-2 and iNOS were determined by immunofluorescence staining. DAPI-stained nuclei were indicated by blue fluorescence. Cox-2 and iNOS were indicated by green fluorescence. Scale Bar = 100 μm. (B) Cells were pretreated with the indicated concentrations of Fucoxanthin for 1 h before being stimulated with LPS (100ng/mL) for another 24 h. The expression levels of COX-2 and iNOS were determined by immunoblotting. *P < 0.05, **P < 0.01 compared with the LPS group.