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. 2021 Feb 15;131(4):e141335. doi: 10.1172/JCI141335

Figure 1. Effect of MG132 on subcellular localization of GFP-tagged AR and AR deletion constructs.

Figure 1

(A) Diagrams of GFP-tagged AR and AR deletion constructs: GFP-AR, GFP-NESAR, GFP-LBD, GFP-ΔNESARLBD, GFP-ΔNESARAR, GFP-NTD, GFP-NTD-LBD, GFP-DBDH, and GFP-DBDH-LBD. (BF) Representative fluorescent images of transfected GFP-NESAR (B), GFP-LBD, GFP-ΔNESARLBD (C), GFP-ΔNESARAR, GFP-AR (D), GFP-NTD, GFP-NTD-LBD (E), GFP-DBDH, and GFP-DBDH-LBD (F) in COS-7 cells 24 hours after treatment with DMSO, CHX, and/or MG132. Con, control. Data represent 1 of 2 independent experiments with consistent results. Original magnification, ×40.