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. 2021 Feb 15;131(4):e141335. doi: 10.1172/JCI141335

Figure 2. Imported nuclear AR is degraded and not exported following DHT withdrawal.

Figure 2

(A) Representative fluorescent images of transfected GFP-AR in COS-7, LNCaP, or C4-2 cells treated with 10 nM DHT followed by DHT withdrawal (W/D) and CHX in presence or absence of MG132. (B) Schematic of ReAsH pulse-chase experiments. (C) Representative fluorescent images of ReAsH pulse-labeled GFP-AR-4Cys. Green signal represented total GFP-AR-4Cys protein, whereas red signal was derived from ReAsH-labeled GFP-AR-4Cys protein. Original magnification, ×40.(D) Schematic of Click pulse-chase analysis. (E and F) Western blot detection of the pulsed labeled protein in cells cultured in the absence (E) or presence (F) of MG132, with (DHT) or without (W/D) DHT. Data represent 1 of at least 2 independent experiments with consistent results.