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. 2021 Feb 15;131(4):e140196. doi: 10.1172/JCI140196

Figure 3. PD-L1 expression of lymphocyte subsets is increased during the resolution of acute liver injury.

Figure 3

Hepatic nonparenchymal cells were isolated from livers of baseline (control) and APAP-treated (24 h, 48 h, or 72 h) WT mice. Phenotypic characterization of liver CD45+ leukocytes was done by flow cytometry. (A) Representative flow cytometric gating strategy used to identify CD4+ Τ cells, CD8+ Τ cells, Tregs, NK cells, and NKT cells. (B) Number of CD4+ T cells (blue), CD8+ T cells (gray), and Tregs (red) per gram of tissue (n = 8–12 per group). (C) Number of NK (blue) and NKT (red) cells per gram of tissue (n = 8–12 per group). (D) Representative histograms and data showing PD-L1 expression (MFI) of CD4+ T cells, CD8+ T cells, and Tregs (n = 4–8 per group). (E) Representative histograms and data showing PD-L1 expression (MFI) of NK and NKT cells (n = 4–8 per group). Results are from 3 (B and C) and 2 (D and E) independent experiments. Each symbol represents an individual mouse. Data are presented as the mean ± SEM. *P < 0.05 and **P < 0.01, by 1-way ANOVA (compared with control).