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. 2021 Feb 15;131(4):e135465. doi: 10.1172/JCI135465

Figure 4. MAPK4 induces GATA2/AR expression and AR activation in PCa cells.

Figure 4

Western blots on (A) LNCaP cells with overexpression of MAPK4, control (Ctrl), Dox-inducible overexpression of MAPK4 (iMAPK4) or control (iCtrl), (B) LAPC4 cells, and (C) 22Rv1 cells with Dox-inducible overexpression of MAPK4 (iMAPK4) or control (iCtrl). Additional loading control (β-ACTIN) was used for the ectopic MAPK4 expression in 22Rv1-iMAPK4 cells. The left and right panels of Western blots were on the same samples run at different times. qPCR on (D) LNCaP, (E) LAPC4, and (F) 22Rv1 cells with Dox-inducible overexpression of MAPK4 (iMAPK4) or control (iCtrl). (G) LNCaP-iMAPK4 cells and (H) LAPC4-iMAPK4 cells were treated with 10 μM MDV3100, vehicle control (Ctrl), or increasing doses (up to 10 nM) of R1881 in 10% of CSS in the presence (+) or absence (–) of 0.5 μg/mL Dox. qPCR was used for assessing PSA expression. Data represent mean ± SD. P values determined by unpaired 2-tailed Student’s t test. ***P ≤ 0.001. ****P ≤ 0.0001. Data are representative of at least 3 independent experiments.