Skip to main content
. 2021 Feb 15;131(4):e140333. doi: 10.1172/JCI140333

Figure 3. Inhibition of Pol II phosphorylation by E. coli 83972 single gene mutants.

Figure 3

(A) Schematic of genomic changes detected in the E. coli SN25 genome (red lines) compared with the ancestral strain E. coli 83972 (black lines) (n = 48). Genes with mutations leading to amino acid changes or frame shifts (fs) are indicated in red. (B and C) Pol II inhibition by E. coli 83972 single gene deletion mutants. Human kidney epithelial cells were infected for 4 hours, stained with antibodies against Pol II phosphorylated on Ser2 (Pol II-p), and analyzed by (B) confocal microscopy or (C) flow cytometry. The inhibitory phenotype of E. coli 83972 was attenuated after deletion of lldD, lldR, nlpD, rfaH, or cysE. (D) Pol II inhibition by supernatants from E. coli 83972 single gene deletion mutants. The ΔlldD, ΔlldR, ΔnlpD, or ΔrfaH deletions (red) attenuated Pol II inhibition, reproducing the E. coli SN25 phenotype. Scale bars: 10 μm. Data are presented as mean ± SEM (n = 2–10 experiments). *P < 0.05, **P < 0.01, ***P < 0.001 compared with E. coli 83972 by Kruskal-Wallis test with Dunn’s multiple-comparison test.