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. 2021 Feb 15;131(4):e135296. doi: 10.1172/JCI135296

Figure 4. Endothelial Sema3G deletion causes a hyperpruned vascular network in growing retinal vessels.

Figure 4

(A and B) Schematic illustration of the developmental stages during retinal angiogenesis and the quantitative indicators. (C) Confocal images showing one-quarter of P5 and P10 flat-mount retinas. Higher magnification images are displayed in the right panel. (D) Comparisons of percentage of vessel area and average vessel length of the blood vessels in retinas at P5 or P10 (n = 6 mice for each group). Error bars represent mean ± SEM. **P < 0.01; ***P < 0.001; 2-tailed Student’s t tests. Scale bars: 500 μm (C); magnified images: 100 μm (C).