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. 2021 Feb 2;17(2):e1009290. doi: 10.1371/journal.ppat.1009290

Fig 5. Expression of IL23A in epithelial cells is much greater in animals infected with WT vs ΔΔstx EHEC.

Fig 5

(A) Micrographs of colon sections from rabbits inoculated with WT EHEC, ΔΔstx EHEC, or PBS (mock) stained with a probe to rabbit IL23A mRNA (red) and DAPI (blue). Scale bar is 500 μM. (B) Percentage of tissue section with IL23A signal from individual colons. Distributions compared using Mann-Whitney U test, p<0.01 (**), n.s. indicates not significant. (C) Mean fluorescent intensity (MFI) from individual colons plotted with mean. Distributions compared using Mann-Whitney U test, p<0.01 (**), n.s. indicates not significant. (D) Percent IL23A signal within E-cadherin positive cells. Distributions compared using the Mann-Whitney U test, p<0.05 (*). (E) Sections stained with a probe to rabbit IL23A mRNA (red), DAPI (blue), and anti-E-cadherin antibody (white). Scale bar is 500 μM. Example immune cell (IC) and epithelial cell (EC) is indicated. (F) Normalized expression of IL23A in HT29 cells infected with WT EHEC, ΔΔstx EHEC, or PBS. Expression levels compared with a Students two-tailed t-test, p<0.001 (***), n.s. indicates not significant.