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. 2021 Feb 12;16(2):e0246580. doi: 10.1371/journal.pone.0246580

Fig 2. Microscopic observation of colonic mucosa and evaluation of colonic lesions formed with chronic oral administration of ethanol in mice.

Fig 2

Eight-week-old mice were orally gavaged daily with 1.0 mL/day of water (Con), 1.0 mL/day of 1.5% (v/v) ethanol (1.5% EtOH), or 1.0 mL/day of 5.0% (v/v) ethanol (5.0% EtOH) for 10 weeks. At weeks 2 and 10 (2 wk and 10 wk, respectively), representative colonic tissue sections were observed microscopically. (A) HE staining images at 400X magnification, (B) TB staining images at 400X magnification, and (C) MUC2 immunohistochemical staining images at 400X magnification. (D) The microscopic lesion scores were evaluated as described in Materials and Methods. The scores are expressed as means ±SD (n = 5). **p<0.01 and ***p<0.001, versus 2 wk Con as assessed by ANOVA with the Tukey-Kramer test. ##p<0.01 for 2 wk 5.0% EtOH versus 10 wk 5.0% EtOH as assessed by the paired t-test.