Fig 5. Effects of chronic oral administration of ethanol on the colonic population of inflammation-associated immune cells in mice as analyzed by flow cytometry.

(A) The proportion of CD4⁺ CD45⁺ RORγt⁺ T cells, (B) CD4⁺ CD45⁺ Fox3⁺ T cells, (C) CD11b⁺ CD45⁺ F4/80⁺ macrophages detected by flow cytometry: (a) 2-wk Con, (b) 2-wk 1.5% EtOH, (c) 2-wk 5.0% EtOH, (d) 10-wk Con, (e) 10-wk 1.5% EtOH, (f) 10-wk 5.0% EtOH. The quantitative analyses data are shown: (D) CD4⁺ CD45⁺ RORγt⁺ T cells, (E) CD4⁺ CD45⁺ Fox3⁺ T cells, (F) CD11b⁺ CD45⁺ F4/80⁺ macrophages by flow cytometry. For (D), (E) and (F), the results are expressed as means ±SD (n = 4). *p<0.05, **p<0.01 and versus 2 wk control, as assessed by ANOVA with the Tukey-Kramer test. ^#p<0.05 for 2 wk versus 10 wk with the same ethanol dosage, as assessed by the paired t-test. For abbreviations for ethanol-administration conditions, see the legend to Fig 2. For experimental details, see Materials and methods.