Table 1. Characterization of mice with chronic oral administration of ethanol.
| Valuea | 2-wk Groupb | |||
| Con | 1.5% EtOH | 5.0% EtOH | p value | |
| (n = 8) | (n = 8) | (n = 8) | ||
| Body weight (g) | 25.2 ± 1.0 | 25.3 ± 0.8 | 24.9 ± 0.6 | ns |
| Liver/body weight ratio | 3.66 ± 0.26 | 3.91 ± 0.21 | 4.35 ± 0.19 | < 0.01 |
| (g/g, %) | ||||
| Hepatic triglyceride | 23.1 ± 5.0 | 28.4 ± 2.1 | 36.6 ± 3.3 | < 0.01 |
| (TG (μg)/liver weight (g)) | ||||
| Plasma AST (U/L) | 36.1 ± 5.7 | 40.5 ± 8.1 | 52.3 ± 7.9 | < 0.01 |
| Plasma ALT (U/L) | 24.3 ± 2.7 | 38.0 ± 6.8 | 56.8 ± 16.6 | < 0.01 |
| Plasma TG (μmol/L) | 0.52 ± 0.15 | 0.63 ± 0.13 | 0.73 ± 0.15 | < 0.05 |
| Valuea | 10-wk Groupb | |||
| Con | 1.5% EtOH | 5.0% EtOH | p value | |
| (n = 8) | (n = 8) | (n = 7) | ||
| Body weight (g) | 26.9 ± 1.5 | 26.7 ± 0.9 | 26.3 ± 0.7 | ns |
| Liver/body weight ratio | 3.70 ± 0.20 | 3.89 ± 0.17 | 4.07 ± 0.18 | < 0.01 |
| (g/g, %) | ||||
| Hepatic triglyceride | 22.1 ± 2.6 | 26.8 ± 2.1 | 30.1 ± 2.2 | < 0.01 |
| (TG (μg) / liver weight (g)) | ||||
| Plasma AST (U/L) | 33.9 ± 4.5 | 39.5 ± 2.7 | 43.4 ± 5.6 | < 0.05 |
| Plasma ALT (U/L) | 22.6 ± 2.7 | 27.1 ± 2.3 | 34.1 ± 4.9 | < 0.01 |
| Plasma TG (μmol/L) | 0.45 ± 0.05 | 0.57 ± 0.07 | 0.65 ± 0.10 | < 0.01 |
a Values of seven to eight independent biological replicates are expressed as the mean ± SD. Data were assessed using ANOVA with Tukey-Kramer test.
b Orally gavaged daily for 2 weeks (2 wk) or 10 weeks (10 wk) with 1.0 mL water (Con), 1.0 mL of 1.5% (v/v) ethanol (1.5% EtOH), or 1.0 mL of 5.0% (v/v) ethanol (5.0% EtOH).
ns, not significant; TG, triglyceride; AST, aspartate aminotransferase; ALT, alanine aminotransferase.