Figure 5.
Cell coverage and cell seeding efficiency on fresh plasma-treated scaffolds under different preincubation and seeding conditions. Fluorescence microscopy images (F-actin, green) of hMSCs in the cross-sections of scaffolds after 24 h of culture, and (A) preincubated with (+)FBS or (B) (−)FBS, and seeded with (+)FBS or (−)FBS. Quantification of cell seeding efficiency on scaffolds (C) preincubated with (+)FBS and (D) preincubated with (−)FBS. Data presented as average ± s.d. and statistical significance performed using two-way ANOVA with Tukey’s multiple comparison test (*p < 0.05; **p < 0.01; ***p < 0.001). Scale bars 1 mm.