Suppression of the SCD enzyme induces the UPR in pancreatic tumor organoids.
Pancreatic tumor spheroids were treated with or without the SCD inhibitor, A939572, for 16 hours in the presence or absence of stearic acid (SA, 18:0) or oleic acid (OA, 18:1). A, mRNAs regulated by PERK-mediated UPR. B-D, Induction of sXbp1 (B), Atf6 (C), and Grp78 mRNAs (D) by A939572. Note that oleic acid, but not stearic acid, suppresses the induction of these genes. (E) Expression of Scd1 and Scd2 mRNAs. Expression values were normalized against Gapdh. **P < 0.01.