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. Author manuscript; available in PMC: 2022 Mar 1.
Published in final edited form as: FASEB J. 2021 Mar;35(3):e21386. doi: 10.1096/fj.202002071R

Figure 5: Transcriptomic analyses identify novel targets of BMP signaling in ECs.

Figure 5:

(A-C) Pairwise comparison of transcripts of which expression was increased in HUVECs expressing BMPR1CA. Comparison of transcripts of which expression was elevated in ALK1CA or ALK2CA expressing HUVECs (A), in ALK1CA or ALK3CA expressing HUVECs (B), and in ALK2CA or ALK3CA expressing HUVECs (C) identified a number of transcripts of which expression was induced by activation of BMPR1s. (D) Venn diagram of transcripts of which expression was elevated in BMPR1CAs. 119 transcripts were identified as ‘shared’ downstream targets of BMPR1s in ECs. (E) Transcripts of which expression was increased by CA-BMPR1 were validated by quantitative RT-PCR in HUVECs expressing ALK1CA, ALK2CA, or ALK3CA. (F) SMAD1/5 binding to the 5′ regulatory region of the newly identified ‘shared’ downstream targets was assessed by previously reported chromatin immunoprecipitation.