Table 3. INSIGHT reaction mixtures.
| Life Sciences reaction mixture (RM) | |||
| Volume | Stock concenctration | Concentration in RM | |
| Saliva lysate | 1 μl | ||
| Primers/beacon mix | 1 μl | 500 nM each primer | 25 nM each primer |
| 400 nM beacon | 20 nM beacon | ||
| Spiked-in viral RNA/water | 3 μl | ||
| Buffer (NECB-24) | 6.7 μl | ||
| Nucleotide (NECN-24) | 3.3 μl | ||
| Enzyme mix (NEC-1-24) | 5 μl | ||
| Total volume | 20 μl | ||
| In-house reaction mixture | |||
| Volume | Stock concentration | Concentration in RM | |
| Saliva lysate | 1 μl | ||
| Primers/beacon mix | 1 μl | 500 nM each primer | 25 nM each primer |
| 400 nM beacon | 20 nM beacon | ||
| Spiked-in viral RNA/water | 4 μl | ||
| Buffer with DMSO* | 5 μl | ||
| Nucleotide mix* | 4 μl | ||
| Enzyme mix* | 5 μl | ||
| Total volume | 20 μl | ||
*See table S1 for detailed composition.