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| Volume |
Stock concentration |
Concentration in RM |
|
| Saliva lysate | 4 μl | ||
| Primers | 4 μl | 500 nM each primer |
25 nM each primer |
| Spiked-in viral RNA/ water |
8.16 μl | ||
| Probe A | 1.92 μl | 1 μM | 24 nM |
| Probe B | 1.92 μl | 1 μM | 24 nM |
| Reconstituted lyophilized reaction buffer mix (LRB) |
40 μl | ||
| Reconstituted lyophilized enzyme mix (LEM) |
20 μl | ||
| Total volume | 80 μl |
