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. 2021 Feb 2;10:e58756. doi: 10.7554/eLife.58756

Figure 4. dilp-6 is expressed in neurons and cortex glia and received by neuropile glia.

Figure 4.

(A and B) Dilp-6GAL4>UAShisYFP cells are mostly Repo+ Pros− glia that do not surround the neuropile (white arrows), and from position appear to be cortex and surface glia. No YFP+ cells have Pros. Some cells are Repo− Pros− Elav+ (yellow arrowheads) meaning they are neurons. (C and D) qRT-PCRs showing that: (C) kon knock-down in glia (konc452/UASkonRNAi; repoGAL4/+) downregulates dilp-6 mRNA levels; (D) overexpression on kon does not cause a significant effect. N = 3 replicates for both. (C and D) One-way ANOVA, only differences in (C) for dilp-6 mRNA significant p=0.0362, *p<0.05. (E and F) inR expression visualised with reporter InRNP2552GAL4> UAShistoneYFP is expressed stochastically in some dorsal Repo+ neurople glia (white arrows), and other glia, and in some Elav+ neurons (yellow arrowheads). (A and E) Horizontal views of the abdominal ventral nerve cord (VNC); (B and F) transverse views. For full genotypes and further statistical analysis details, see Supplementary file 1.