Figure 1. Dichloroacetate (DCA) in combination with metformin (MET) leads to reduced cell growth. A. Concentration response curve of DCA, B. concentration response curve of MET in U-87 cell line. U-87 cells were treated with DCA (1-100 mM) and MET (1-100 mM) and their IC50 values were determined. To evaluate the efficacy of their combinations, data was analyzed using the Calcusyn Software, and combination (CI) values were generated. C. Synergistic cytotoxic effect of DCA and MET, (***p<0.001).