Figure 2. PolyIC induced melanoma differentiation-associated gene 5 (MDA5) expression on cultured human podocytes. qRT-PCR (A, B) and western blot (C, D) analysis showed an increased expression of MDA5 after polyIC treatment. Five hundred ng/ml polyIC up-regulated mRNA of MDA5 (A) and 500 μg/ml PolyIC induced protein of MDA5 (C) in a time-dependent manner. Similarly, polyIC induced mRNA (B) and protein (D) of MDA5 in dose dependent manner. GAPDH was used as an internal control. The results represent the means±SEM (n=3 wells). *p<0.05; **p<0.001 versus control.