Figure 4. Knockdown of RIG-I and MDA5 with siRNA inhibited the expression of inflammatory cytokines by polyIC. Cultured human podocytes were transfected with non-targeting (control) siRNA, RIG-I, and MDA5 siRNA using Dharmafect Reagent®. PolyIC (500 ng/ml) was added and incubated for 6 h. qRT-PCR analysis showed the gene expression levels of IFN-β (A) and IL-6 (B). GAPDH was used as an internal control. The results represent the means±SEM (n=3 wells). *p<0.05; **p<0.001.
