Figure 1. Inhibition of colony-stimulating factor-1 receptor reduces M2 macrophage polarization. Bone marrow derived monocytes were cultured with granulocyte-macrophage colony-stimulating factor, followed by M2 induction with interleukin-4 and -13 with or without BLZ-945. Representative fluorescence-activated sorting plots are shown. Cells were initially gated by the pan-macrophage markers CD45 and F/480 to identify total macrophages. The subset of macrophages that stained for CD11b and CD206 were then selected to identify M2 macrophages. Experiments were performed in triplicates.
