Figure 2. Inhibition of colony-stimulating factor-1 receptor potentiates the impact of radiotherapy (RT) on tumor volume. TRP murine glioblastoma cells were stereotactically injected into the right hemisphere of 6- to 8-week-old mice. On day 14, treatment with vehicle, BLZ-945 (200 mg/kg), RT (6 Gy × 3 fractions), or BLZ-945 + RT was initiated, followed by daily treatment with vehicle or BLZ-945 for 2 weeks. A: Representative hematoxylin and eosin (H&E) images from each treatment group. Pink-stained tissue shows normal brain. Blue-stained tissue shows tumor cells. B: Tumor burden expressed as a percentage of normal brain volume based on H&E staining. Brains were harvested on day 10 post-treatment initiation and sectioned every 10 μM through the tumor and adjacent brain tissue. Slides were imaged and segmented. An iterative learning algorithm was employed to identify normal brain tissue and tumor cells. p-Values for significant differences are shown.