Figure 5. Immunophenotyping of the tumor microenvironment. From the survival experiment, brains were harvested at the time of sacrifice, tumors were extracted, and fluorescence-activated sorting was used to analyze the presence of various immune cells in each treatment arm. A: Macrophages, identified as CD45-, CDF4/80-, and CD11b-positive cells. B: M2 macrophages, identified by the markers in (A) in addition to CD206. C: CD8+ T-cells, identified as CD3- and CD8-positive cells. D: M2 to CD8 cell ratio. E: Regulatory T-cells (Tregs), identified as CD4- and CD25-positive cells. p-Values for significant differences are shown.
