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. Author manuscript; available in PMC: 2021 Jun 15.
Published in final edited form as: Oncogene. 2020 Dec 15;40(6):1106–1117. doi: 10.1038/s41388-020-01585-5

Figure 7. Homodimerization and DNA binding, but not dimerization with AR-FL, is required for AR-V7 to mediate DNA-damage repair.

Figure 7.

Evaluation for double-stranded DNA breaks using γ-H2AX foci in LNCaP cells stably expressing cumate-inducible FLAG-tagged AR-V7WT or AR-V7MT construct at 0, 0.5, or 8 hr after 2 Gy ionizing irradiation under androgen-deprived condition showing that AR-V7F, but not AR-V7D, AR-V7FD, or AR-V7P, retains the ability to mediate DNA-damage repair. A. Schematic of the experiment and images of γ-H2AX foci within the cells. B. Quantification of more than 50 nuclei per condition for the average number of γ-H2AX foci per cell. *, P < 0.01.