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. Author manuscript; available in PMC: 2021 Jul 18.
Published in final edited form as: Nat Cell Biol. 2021 Jan 18;23(2):160–171. doi: 10.1038/s41556-020-00624-3

Fig. 5. ALC1 function in base damage repair is not epistatic with HR and single-strand break repair (SSBR).

Fig. 5

a, Sensitivities of the indicated DLD1 isogenic lines to methyl methanesulfonate (MMS) and camptothecin (CPT) in the CellTiter-Glo assay. Data are mean ± s.e.m. from n = three biologically independent experiments. b, Schematic of SSBR pathway. While base damage requires processing events at the chromatin, CPT traps TOP1 on already nicked substrates. Replication of damaged bases results in gaps and SSBs, that increase reliance on HR for repair. c, Sensitivities of the indicated DLD1 cell lines to MMS and CPT in the CellTiter-Glo assay. Data are mean ± s.e.m. from n = three biologically independent experiments. d, Schematic and quantification of fiber length in the absence and presence of S1 nuclease in the indicated cell lines. Median is indicated. p-value determined by Mann-Whitney was derived from n≥75 fibers sampled over two independent experiments. Cells were incubated with 100 nM ola for 24 hrs. Source data are provided.