Table 1.

Composition and characteristics of the two triplex qPCR assays. Assay set 1 targets abundant, high proteolytic Pseudomonas species, and set 2 detects abundant, but less proteolytic species and total pseudomonads. Sequences of six primer pairs (Pr_F and Pr_R) and hydrolysis probes (P1–P6), final concentrations, amplicon length, target genes, and target species are listed. Probes’ fluorophores (5′-ends) and quenchers (3′-ends) are shown in bold

Primer/probe	Sequence (5′-3′)	Conc. [nM]	Amplicon length [bp]	Target gene	Target species
Assay set 1
Pr1_ F	GCACCAATGASAAGTACCACA	400	135	aprX	P. proteolytica, P. gessardii, P. gessardii-like
Pr1_R	GTATGGCCGATCTCGTGG	600
P1	CY5-CACGGATGGCACCTCGTGGTAC-BHQ2	200
Pr2_F	ACCTTCCTCACCTCGGCT	600	137	aprX	P. fluorescens, P. lactis, P. lactis-like
Pr2_R	GGTAAAGGTCACGTTGGCA	600
P2	TexasRed-AACACCCAGCAGAAAGCACAGGC-BHQ2	200
Pr3_F	GCATCTGCCGAACAACAAC	400	85	aprX	P. protegens, P. protegens-like
Pr3_R	CGATCGTATTGGTGGCTGA	200
P3	FAM-CCGCAGCAAGTTCGGCGTATAAC-BHQ1	150
Assay set 2
Pr4_F	AGCAGCATTGTCCGTTGG	400	130	aprX	P. fragi
Pr4_R	CGGTGGTGAGCGAAGGT	600
P4	FAM-CGGCAAACACCGGCAGTTCTG-BHQ1	200
Pr5_F	TGCTGGCCTGGTTGTAGC	600	92	aprX	P. lundensis, P. lundensis-like
Pr5_R	TCACCGGGATTACTCATCTCA	600
P5	TexasRed-ACGACCGCATCACCCGCCT-BHQ2	200
Pr6_F	CAGCCGYTGGGTGGTAA	400	130	rpoB	Pseudomonas spp.
Pr6_R	CCGTTCACATCGTCCGA	200
P6	CY5-AGTTCGGTGGTCAGCGTTTCGG-BHQ2	150