Table 3.

C_q values from triplex qPCR assays, applying five species-specific probes (P1–P5) and the universal Pseudomonas probe (P6). Genomic DNA from 26 target strains was used as template in a final concentration of 0.1–0.2 ng/μl. Mean values of two technical replicates per measurement are shown. Hyphens (-) represent no signal in qPCR. C_q values above the defined threshold of 33 were considered as not quantifiable and are given in brackets

	Species-specific hydrolysis probes					Universal Pseudomonas probe
Target Strains (P1–P5)	P1	P2	P3	P4	P5	P6
Pseudomonas gessardii WS 5049P1	21.05	-	-	-	-	20.70
Pseudomonas gessardii WS 5408P1	21.05	-	-	-	-	19.52
Pseudomonas gessardii WS 5409P1	21.02	-	-	-	-	19.73
Pseudomonas proteolytica WS 5127P1	20.08	-	-	-	-	20.51
Pseudomonas proteolytica WS 5128P1	19.68	-	-	-	-	20.16
Pseudomonas sp. 10 WS 5059P1	19.59	-	-	-	-	19.89
Pseudomonas sp. 9 WS 5411P1	20.46	-	-	-	-	20.41
Pseudomonas sp. 9 WS 5412P1	20.86	-	-	-	-	19.95
Pseudomonas sp. 9 WS 5413P1	20.67	-	-	(34.81)	-	20.51
Pseudomonas fluorescens DSM 50090T,P2	-	22.13	-	-	-	20.83
Pseudomonas fluorescens WS 5037P2	-	20.32	-	-	-	20.00
Pseudomonas lactis DSM 29167T,P2	-	20.64	-	-	-	19.48
Pseudomonas lactis WS 5000P2	-	20.03	-	-	-	19.06
Pseudomonas lactis WS 5404P2	-	21.86	-	-	-	20.39
Pseudomonas lactis WS 5405P2	-	21.72	-	-	-	19.73
Pseudomonas sp. 12 DSM 29142P2	-	21.05	-	-	-	20.61
Pseudomonas sp. 12 WS 5407P2	-	21.27	-	-	-	20.53
Pseudomonas protegens WS 5082P3	-	-	21.59	-	-	20.25
Pseudomonas protegens WS 5415P3	-	-	20.56	-	-	19.14
Pseudomonas sp. 5 WS 5414P3	-	-	20.48	-	-	19.12
Pseudomonas fragi WS 5094P4	(33.26)	-	-	19.35	-	19.00
Pseudomonas fragi WS 5112P4	(33.46)	-	-	18.85	-	18.53
Pseudomonas fragi WS 5416P4	-	-	-	-	-	19.13
Pseudomonas lundensis WS 5024P5	-	-	-	-	18.57	19.00
Pseudomonas lundensis WS 5095P5	-	-	-	-	18.86	19.29
Pseudomonas sp. 6 WS 5071P5	(33.47)	-	-	-	20.00	18.44

^{P1,P2,P3,P4,P5}: target strain of probe P1–P5. ^T: type strain