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. 2021 Feb 12;12:1011. doi: 10.1038/s41467-021-21366-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Percentages (in y) of wildtype (WT), CTCF^KO and animals lacking CTCF in neural stem cells (NSCs), neurons or muscle that successfully transition from third instar larva to pupa (black) and from pupa to adult (gray) in n = 3 biological replicates (rep 1–3), each containing the indicated number of animals per genotype. Horizontal lines show means. (b) Percentage of live adults of the same genotypes up to 30 days after pupal hatching. 40 animals of each genotype were analyzed in triplicate; dark lines show mean and shading shows ±standard deviation. c Same as (a) but for WT, CTCF⁰ and animals with CTCF expression restricted to NSCs, neurons or muscle. d Same as (b) but for genotypes indicated in (c).