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. 2021 Feb 12;4:191. doi: 10.1038/s42003-021-01707-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Schematic representation of the histone exchange assay. The NCP was incubated with the H2A-H2B dimer, in which the H2B protein was conjugated with the Alexa Fluor 488 fluorescent dye (H2A-H2B^fluo dimer). The samples were analyzed by native-PAGE. b–d Representative gel images of the histone exchange assay. The H2A NCP (1 µM) was incubated with the H2A-H2B^fluo dimer (0 µM: lane 1; 2 µM: lanes 2, 3, and 4) for 10 (lane 2), 120 (lane 3), and 360 (lanes 1 and 4) minutes. The H2A.B NCP (1 µM) was incubated with the H2A-H2B^fluo dimer (0 µM: lane 5; 2 µM: lanes 6, 7, and 8) for 10 (lane 6), 120 (lane 7), and 360 (lanes 5 and 8) minutes. After the incubation, the samples were analyzed by native-PAGE. The gels were visualized by ethidium bromide staining, or through the Alexa488 conjugated with the H2A-H2B dimer. b Ethidium bromide; c Alexa488 (H2A-H2B^fluo); d Overlay of ethidium bromide (green) and Alexa-488 (magenta). The asterisk indicates the NCP-histone complexes. Reproducibility is confirmed by three independent experiments, and the results are presented in Supplementary Fig. 3. The uncropped gel images are shown in Supplementary Fig. 9.