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. 2021 Feb 12;4:191. doi: 10.1038/s42003-021-01707-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, b Sequential HS-AFM images of the H2A.B NCP (a) and the H2A nucleosome (b). Representative NCPs with about 6 nm heights are shown, and the dissociation processes of the NCPs elicited by scratching with the HS-AFM probe were monitored. White arrows indicate possible histone dimers. Scale bars are 20 nm. Frame rate is 2.5 frames per second.