Skip to main content
. 2021 Feb 12;12:1003. doi: 10.1038/s41467-021-21162-y

Fig. 2. Wnt-independent growth of organoids after infection with pks+ E. coli.

Fig. 2

a Organoids formed after seeding 50,000 non-infected, pks+ WT E. coli-infected, and E. coli ΔclbR-infected cells from three independent replicates. Data represent mean ± SD, p < 0.05, as calculated by two-sided unpaired Student’s t-test. b Scheme describing the infection of organoids. c Organoids grow without the addition of Wnt and CHIR99021 to the medium for the pks+ WT E. coli-infected condition, but not for the non-infected or E. coli ΔclbR-infected conditions. Image representative of four independent replicates. Scale bars: 1 mm. d Organoids formed after seeding 10,000 cells in each condition. Image representative of three independent replicates. Scale bars: 1 mm. e Quantification of the number of organoids formed after seeding 10,000 cells in each condition from three replicates. Data represent mean ± SD, p < 0.05, as calculated by two-sided unpaired Student’s t-test. f Wnt-independent organoids have a higher splitting ratio and grow indefinitely when compared to non-infected organoids. Graph representative of four independent replicates. g Immunofluorescence staining for Ki67 (red) in non-infected organoids and Wnt-independent organoids. Hoechst (blue) stains for DNA. Image representative of three independent replicates. Scale bars: 100 μm. h Quantification of the percentage of Ki67-positive cells from three replicates. Data represent mean ± SD, p < 0.05, as calculated by two-sided unpaired Student’s t-test. NI = non-infected and WI = Wnt-independent. Source data are provided as a Source Data file.