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. 2021 Feb 12;11:3753. doi: 10.1038/s41598-021-83081-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Boxplot of MetaCompare risk scores by individual sample, where influent is represented as squares and activated sludge is represented as circles. The box inner line represents the median while the whiskers represent the 25th and 75th percentiles. (b) NMDS plot of annotated of ARGs, MGEs, and pathogen gene marker profiles underlying MetaCompare risk scores. Where CHE Switzerland, HKG Hong Kong, IND India, SWE Sweden, USA United States of America. Assembler type, treatment stage and sample were significant factors in system clustering (ANOSIM; R = 0.26, p = 0.001; R = 0.26, p = 0.001; R = 0.33, p = 0.001). This figure was generated using the ggplot2(v3.3.0) package in R(v3.5.0).