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. 2021 Feb 12;11:3753. doi: 10.1038/s41598-021-83081-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Assembler performance in assembling the in silico spiked genome of Marinobacter hydrocarbonoclasticus ATCC 49840 into two metagenomic samples (where circles represent USA-AS and squares represent USA-Inf). (a) Misassemblies per million basepairs within contigs aligning to the M. hydrocarbonoclasticus ATCC 8 genome. (b) Ratio of contigs with misassemblies to total contigs aligning to the M. hydrocarbonoclasticus genome. Y-axis indicates sequencing coverage, reported as long by short read coverages for hybrid assemblers. Hybrid assembly coverage is labeled with the long-read coverage by short-read coverage. This figure was generated using the ggplot2(v3.3.0) package in R(v3.5.0).