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. 2021 Feb 12;11:3753. doi: 10.1038/s41598-021-83081-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Circular depictions of the Marinobacter hydrocarbonoclasticus ATCC 49,840 genome with assembler type from USA-AS samples. (a) MEGAHIT with 1 × , 5 × , 10 × , 50×, alternating between misassemblies (pink) and correct assemblies (blue). (b) metaSPAdes with 1 × , 5 × , 10 × , 50×. (c) IDBA-UD with 1 × , 5 × , 10 × , 50×. (d) metaFlye with 0.1 × , 1 × , and 5 × coverage. (d) Canu with 1 × and 5 × coverage (no Flye contigs mapped at 0.1 × coverage). (e) Canu with 0.1 × , 1 × , and 5 × coverage. (d) Canu with 1 × and 5 × coverage (no Flye contigs mapped at 0.1 × coverage). (f) OPERA-MS with 10 × short-read and 0.1 × , 1 × , and 5 × long-read coverage. (g) HybridSpades with 10 × short-read and 0.1 × , 1 × , and 5 × long-read coverage. Labels are regions of the genome annotated with ARGs or MGE genes. Internal red/black dots are GC skew of 1000 basepair sections of the genome (red is positive GC skew while red–black is negative GC skew). This figure was generated using the Circlize(v0.4.11) package in R(v3.5.0).