Table 1.
Characteristics of selected 29 IgG linear epitopes for detection of SARS-CoV-2 infection.
| S. no. | Epitope ID. | Epitope sequence | Epitope length | AA (start) | AA (end) | Protein name (Acc. MN908947) | Severe COVID-19 Early (N = 22) | Severe COVID-19 Late (N = 22) | Mild- COVID-19 Early (N = 22) | Mild-COVID-19 Late (N = 22) | Asymptomatic COVID-19 (N = 12) | SARS-CoV-1 (N = 11) | Healthy controls (N = 10) | Other HCoV (N = 11) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | OP1 | TAYNGYLTSSSKTP | 13 | 1484 | 1497 | ORF1ab polyprotein (pp1ab) | 5/22 (23%) | 4/22 (19%) | 5/22 (23%) | 5/22 (23%) | 0/12 (0%) | 1/11 (10%) | 0/10 (0%) | 1/11 (10%) |
| 2 | OP2 | LVPNQPYPNASFDNF | 14 | 1914 | 1928 | 8/22 (37%) | 12/22 (55%) | 3/22 (14%) | 3/22 (14%) | 0/12 (0%) | 0/11 (0%) | 0/10 (0%) | 0/11 (0%) | |
| 3 | OP3 | NRFTTTLNDFNLVAMK | 15 | 3484 | 3499 | 1/22 (5%) | 1/22 (5%) | 4/22 (19%) | 3/22 (14%) | 0/12 (0%) | 0/11 (0%) | 0/10 (0%) | 0/11 (0%) | |
| 4 | OP4 | QGLLPPKNSIDAFKLNIK | 17 | 3826 | 3843 | 5/22 (23%) | 5/22 (23%) | 4/22 (19%) | 6/22 (28%) | 0/12 (0%) | 0/11 (0%) | 0/10 (0%) | 2/11 (19%) | |
| 5 | OP5 | NTCVGSDNVTDFNAIATCDW | 19 | 5419 | 5438 | 5/22 (23%) | 13/22 (60%) | 5/22 (23%) | 6/22 (28%) | 0/12 (0%) | 0/11 (0%) | 0/10 (0%) | 0/11 (0%) | |
| 6 | OP6 | FYSYATHSDKFTDGV | 14 | 6292 | 6306 | 7/22 (32%) | 11/22 (50%) | 0/22 (0%) | 2/22 (10%) | 0/12 (0%) | 0/11 (0%) | 0/10 (0%) | 0/11 (0%) | |
| 7 | SP1 | TESNKKFLPFQQFGRDIAD | 18 | 553 | 571 | Surface glycoprotein | 4/22 (19%) | 15/22 (69%) | 1/22 (5%) | 9/22 (41%) | 3/12 (25%) | 0/11 (0%) | 0/10 (0%) | 0/11 (0%) |
| 8 | SP2 | TDAVRDPQTLEILDITPCS | 18 | 573 | 591 | 6/22 (28%) | 17/22 (78%) | 1/22 (5%) | 11/22 (50%) | 2/12 (17%) | 0/11 (0%) | 0/10 (0%) | 0/11 (0%) | |
| 9 | SP3 | QVAVLYQDVNCTEVPVAIHADQLTPTW | 26 | 607 | 633 | 0/22 (0%) | 9/22 (41%) | 2/22 (10%) | 4/22 (19%) | 0/12 (0%) | 0/11 (0%) | 0/10 (0%) | 0/11 (0%) | |
| 10 | SP4 | CASYQTQTNSPRRARSV | 16 | 671 | 687 | 2/22 (10%) | 4/22 (19%) | 0/22 (0%) | 1/22 (5%) | 2/12 (17%) | 0/11 (0%) | 0/10 (0%) | 0/11 (0%) | |
| 11 | SP5 | ARSVASQSIIAYTMSLGAENSVA | 22 | 684 | 706 | 2/22 (10%) | 6/22 (28%) | 4/22 (19%) | 4/22 (19%) | 2/12 (17%) | 0/11 (0%) | 0/10 (0%) | 0/11 (0%) | |
| 12 | SP6 | RALTGIAVEQDKNTQEVFAQV | 20 | 765 | 785 | 11/22 (50%) | 14/22 (64%) | 2/22 (10%) | 5/22 (23%) | 2/12 (17%) | 0/11 (0%) | 0/10 (0%) | 0/11 (0%) | |
| 13 | SP7 | FAQVKQIYKTPPIKDFGGFNF | 20 | 782 | 802 | 5/22 (23%) | 8/22 (37%) | 1/22 (5%) | 3/22 (14%) | 2/12 (17%) | 0/11 (0%) | 0/10 (0%) | 0/11 (0%) | |
| 14 | SP8 | NFSQILPDPSKPSKRS | 15 | 801 | 816 | 1/22 (5%) | 4/22 (19%) | 0/22 (0%) | 4/22 (19%) | 2/12 (17%) | 0/11 (0%) | 0/10 (0%) | 0/11 (0%) | |
| 15 | SP9 | KRSFIEDLLFNKVT | 13 | 814 | 827 | 5/22 (23%) | 8/22 (37%) | 4/22 (19%) | 5/22 (23%) | 1/12 (9%) | 0/11 (0%) | 0/10 (0%) | 0/11 (0%) | |
| 16 | SP10 | PELDSFKEELDKYFKNHTSPDVD | 22 | 1143 | 1165 | 15/22 (69%) | 21/22 (96%) | 9/22 (41%) | 18/22 (82%) | 8/12 (67%) | 1/11 (10%) | 0/10 (0%) | 1/11 (10%) | |
| 17 | SP11 | SPDVDLGDISGINASVV | 16 | 1161 | 1177 | 10/22 (46%) | 20/22 (91%) | 6/22 (28%) | 13/22 (60%) | 0/12 (0%) | 1/11 (10%) | 2/10 (20%) | 1/11 (10%) | |
| 18 | ORF3-1 | EHDYQIGGYTEKWESGVKDCVVL | 22 | 181 | 203 | ORF3a protein | 4/22 (19%) | 7/22 (32%) | 1/22 (5%) | 2/22 (10%) | 0/12 (0%) | 0/11 (0%) | 0/10 (0%) | 0/11 (0%) |
| 19 | MP1 | CDIKDLPKEITVATS | 14 | 159 | 173 | Membrane glycoprotein | 0/22 (0%) | 8/22 (37%) | 0/22 (0%) | 6/22 (28%) | 0/12 (0%) | 0/11 (0%) | 0/10 (0%) | 0/11 (0%) |
| 20 | MP2 | QRVAGDSGFAAYSRY | 14 | 185 | 199 | 0/22 (0%) | 10/22 (46%) | 0/22 (0%) | 4/22 (19%) | 0/12 (0%) | 0/11 (0%) | 0/10 (0%) | 0/11 (0%) | |
| 21 | ORF8-1 | GSKSPIQYIDIGNYTVSCLPF | 20 | 66 | 86 | ORF8 protein | 3/22 (14%) | 12/22 (55%) | 4/22 (19%) | 3/22 (14%) | 0/12 (0%) | 0/11 (0%) | 0/10 (0%) | 1/11 (10%) |
| 22 | NP1 | ALLLLDRLNQLESKMSG | 16 | 220 | 236 | Nucleocapsid phosphoprotein | 3/22 (14%) | 5/22 (23%) | 2/22 (10%) | 5/22 (23%) | 2/12 (17%) | 0/11 (0%) | 0/10 (0%) | 0/11 (0%) |
| 23 | NP2 | NQLESKMSGKGQQQQGQTVTKKSA | 23 | 228 | 251 | 12/22 (55%) | 18/22 (82%) | 8/22 (37%) | 15/22 (69%) | 7/12 (59%) | 0/11 (0%) | 0/10 (0%) | 0/11 (0%) | |
| 24 | NP3 | QTVTKKSAAEASKKPRQK | 17 | 244 | 261 | 2/22 (10%) | 7/22 (32%) | 1/22 (5%) | 4/22 (19%) | 2/12 (17%) | 0/11 (0%) | 0/10 (0%) | 0/11 (0%) | |
| 25 | NP4 | RGPEQTQGNFGDQELI | 15 | 277 | 292 | 6/22 (28%) | 3/22 (14%) | 2/22 (10%) | 3/22 (14%) | 2/12 (17%) | 0/11 (0%) | 0/10 (0%) | 0/11 (0%) | |
| 26 | NP5 | AYKTFPPTEPKKDKKKK | 16 | 359 | 375 | 2/22 (10%) | 5/22 (23%) | 0/22 (0%) | 2/22 (10%) | 2/12 (17%) | 0/11 (0%) | 0/10 (0%) | 0/11 (0%) | |
| 27 | NP6 | KDKKKKADETQALPQR | 15 | 370 | 385 | 3/22 (14%) | 7/22 (32%) | 0/22 (0%) | 5/22 (23%) | 3/12 (25%) | 0/11 (0%) | 0/10 (0%) | 0/11 (0%) | |
| 28 | NP7 | ADETQALPQRQKKQQTVTLLPAADL | 24 | 376 | 400 | 4/22 (19%) | 17/22 (78%) | 4/22 (19%) | 9/22 (41%) | 2/12 (17%) | 0/11 (0%) | 0/10 (0%) | 0/11 (0%) | |
| 29 | NP8 | TLLPAADLDDFSKQLQQSMSSADS | 23 | 393 | 416 | 14/22 (64%) | 22/22 (100%) | 6/22 (28%) | 18/22 (82%) | 7/12 (59%) | 1/11 (10%) | 0/10 (0%) | 0/11 (0%) |
Sequences (aa), length, aa location in protein is based on proteome of Severe acute respiratory syndrome coronavirus 2 isolate Wuhan-Hu-1 (accession no. MN908947).