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. 2021 Feb 12;11:3718. doi: 10.1038/s41598-021-82686-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of LGALS3 and CA125 (MUC16) in human ovarian cancers. Three anonymized human tissue microarrays were constructed by collecting 1–3 cores from paraffin embedded, fixed blocks, as previously described²⁷. (A) Examples of human tumor cores stained for CA125 (panel i) and Gal3 (panel ii). Examples of core that did not stain for CA125 (panel iv) and tumor that did not stain for Gal3 (panel iii). (B) Each core was qualitatively scored for prevalence of CA125 and Gal3 positive cells (0 = < 5%, 1 + = 5–25%; 2 + = 25–50%; 3 + = 50–75% and 4 + = > 75%. The intensity for Gal3 was separately scored as low, medium and high (represented by green, orange and red dots respectively). All cores were independently scored by a blinded reference pathologist (KP).